Category Archives: uncategorized

Post ASM Conference reflections

Contributed by Darwin Bandoy, DVM I participated in the 2018 ASM Conference on Rapid Applied Microbial Next-Generation Sequencing and Bioinformatic Pipelines, September 23–26, 2018, in Tysons, Virginia and presented my work in the lab using next-generation sequencing of Campylobacter hyointestinalis. … Continue reading

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Pan-Genome Analysis with Roary

Contributed by Shawn Higdon Microbial Genomics involves the isolation of microbes from environmental samples, often times with the intent of generating a pure culture comprised by an organism of a single type of strain. While many isolation events lead to … Continue reading

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Diabetes and Gut Microbiome Study

Contributed by Carol Huang Metabolic disease diabetes and obesity have impacted quite some percentage of the population, over 600 million people in the world are obese, and over 400 million have diabetes. Type I diabetes often involves the loss of … Continue reading

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Reproducible Bioinformatic Workflows with Snakemake

Contributed by Shawn Higdon As I begin the newest chapter of my PhD journey – a cross-college internship at UC Davis with the Weimer lab in School of Veterinary Medicine – I am faced with new computational tasks that demand … Continue reading

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Non-mammals and mammal gut defense

Contributed by Nguyet Kong Chitin is a fibrous substance containing polysaccharides and form the exoskeleton of arthropods such as insects and crustaceans and are commonly found on the cell walls of fungi. Non- mammals protect their gut walls with a … Continue reading

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Is there only one way to fix atmospheric nitrogen?

Contributed by Shawn Higdon Biological Nitrogen fixation energy-intensive process carried out by Bacteria and Archaebacteria that have been classified across many phyla. While there is an overwhelming amount of research that describes and characterizes the process of biological nitrogen fixation … Continue reading

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Listeria Outbreak in Frozen Vegetables in Europe

Contributed by Nguyet Kong A recent Listeria outbreak in Europe has been reported due to the insufficient cooking of frozen vegetables, which led to the question if frozen vegetables even safe to eat at all. The European Food Agency is reminding people … Continue reading

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Rapid Analysis of Microbial Growth Curve Data in R

Contributed by Shawn Higdon As a graduate student at the interface of plant biology and microbiology working with pure isolates, analyzing microbial growth curve data is unavoidable. I was recently in a situation where I needed to calculate the slope … Continue reading

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Bacillus: The Dark Side vs. The Good Side

Contributed by Darwin Bandoy, DVM This particular talk by Bill Widner of Novozymes tantalized my interest as I only associate Bacillus with things a veterinarian must know to avoid, which is Bacillus anthracis, a deadly disease causing gram-positive bacteria in … Continue reading

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Assessing Contamination of Whole Genome Sequences for Microbial Isolates

Contributed by Shawn Higdon You are working tirelessly in the lab attempting to generate cultures of microbes that are pure, meaning a single organism comprising a single well-isolated colony. You finally have what appears to be an isolate of pure … Continue reading

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